phenotypic.prefab.HeavyOtsuPipeline#

class phenotypic.prefab.HeavyOtsuPipeline(gaussian_sigma: int = 5, gaussian_mode: str = 'reflect', gaussian_truncate: float = 4.0, otsu_ignore_zeros: bool = True, otsu_ignore_borders: bool = True, mask_opener_footprint: Literal['auto'] | ndarray | int | None = 'auto', border_remover_size: int = 1, small_object_min_size: int = 50, texture_scale: int = 5, texture_warn: bool = False, benchmark: bool = False, verbose: bool = False)[source]#

Bases: PrefabPipeline

Detect and measure colonies using multi-stage Otsu thresholding with refinement.

A robust general-purpose pipeline that chains preprocessing, Otsu detection, morphological cleanup, grid alignment, and re-detection for reliable colony segmentation on standard grid plates.

Steps:

GaussianBlur — smooth noise
EnhanceLocalContrast — boost local contrast
MedianFilter — remove residual speckle
FocusEdgeSobel — enhance colony edges
OtsuDetector — threshold-based detection
MaskOpening — smooth mask boundaries
RemoveBorderObjects — remove partial edge colonies
SmallObjectRemover — remove noise fragments
MaskFill — fill interior holes
GridOversizedObjectRemover — remove merged multi-well objects
ReduceSectionsByLine — keep one colony per well
GridAligner — straighten the grid

Measurements: MeasureShape, MeasureColor, MeasureTexture, MeasureIntensity.

Best For:

Standard 96-well or 384-well yeast plates with clean backgrounds.
General-purpose colony detection when you are unsure which detector to use.
Plates with uniform illumination and bimodal intensity histograms.

Consider Also:

HeavyWatershedPipeline when colonies touch or overlap.
RoundPeaksPipeline for a faster, lighter approach on well-separated round colonies.
FilamentousFungiPipeline for filamentous organisms.

See also

Tutorial 8: Using Prefab Pipelines for a visual comparison of prefab pipelines. Prefab Pipelines: Which One for Which Organism for guidance on choosing a prefab pipeline.

Methods

`__init__`	Initializes the object with a sequence of operations and measurements for image processing.
`analyze`	Run the analysis chain (filters then model) against an aggregate frame.
`apply`	The class provides an interface to process and apply a series of operations on an Image.
`apply_and_measure`	Applies processing to the given image and measures the results.
`apply_napari`	Apply the pipeline and progressively add layers to a napari viewer.
`apply_with_intermediates`	Apply the pipeline and capture a snapshot of the image after each operation.
`benchmark_results`	Return execution times and memory usage for operations and measurements.
`construct`
`copy`	Returns a copy of the model.
`dict`
`from_json`	Deserialize a PrefabPipeline from JSON.
`from_orm`
`get_filters`	Get a copy of the analysis filter chain.
`get_meas`	Get a copy of the measurements dictionary.
`get_model`	Get the analysis endpoint model, if configured.
`get_ops`	Get a copy of the operations dictionary.
`get_post`	Get a copy of the post-measurement transforms dictionary.
`get_qc`	Get a copy of the QC config entry list.
`json`
`measure`	Measures properties of a given image and optionally includes metadata.
`model_construct`	Creates a new instance of the Model class with validated data.
`model_copy`	!!! abstract "Usage Documentation"
`model_dump`	!!! abstract "Usage Documentation"
`model_dump_json`	!!! abstract "Usage Documentation"
`model_json_schema`	Generates a JSON schema for a model class.
`model_parametrized_name`	Compute the class name for parametrizations of generic classes.
`model_post_init`	Initialize logging and memory tracking after model construction.
`model_rebuild`	Try to rebuild the pydantic-core schema for the model.
`model_validate`	Validate a pydantic model instance.
`model_validate_json`	!!! abstract "Usage Documentation"
`model_validate_strings`	Validate the given object with string data against the Pydantic model.
`parse_file`
`parse_obj`
`parse_raw`
`schema`
`schema_json`
`set_filters`	Set the analysis filter chain.
`set_meas`	Sets the measurements to be used for further computation.
`set_model`	Set the analysis endpoint model.
`set_ops`	Sets the operations to be performed.
`set_post`	Set the post-measurement transforms.
`set_qc`	Set the QC config entry list.
`to_json`	Serialize the pipeline configuration to JSON format.
`update_forward_refs`
`validate`
`widget`	Return (and optionally display) the root widget.

Attributes

`desc`	Get pipeline description.
`model_computed_fields`
`model_config`	Configuration for the model, should be a dictionary conforming to [ConfigDict][pydantic.config.ConfigDict].
`model_extra`	Get extra fields set during validation.
`model_fields`
`model_fields_set`	Returns the set of fields that have been explicitly set on this model instance.
`name`
`desc_value`
`benchmark`
`verbose`
`reset`
`nrows`
`ncols`
`ops`
`meas`
`post`
`filters`
`model`
`qc`

Parameters:

gaussian_sigma (int)
gaussian_mode (str)
gaussian_truncate (float)
otsu_ignore_zeros (bool)
otsu_ignore_borders (bool)
mask_opener_footprint (Literal['auto'] | ~numpy.ndarray | int | None)
border_remover_size (int)
small_object_min_size (int)
texture_scale (int)
texture_warn (bool)
benchmark (bool)
verbose (bool)

__init__(gaussian_sigma: int = 5, gaussian_mode: str = 'reflect', gaussian_truncate: float = 4.0, otsu_ignore_zeros: bool = True, otsu_ignore_borders: bool = True, mask_opener_footprint: Literal['auto'] | ndarray | int | None = 'auto', border_remover_size: int = 1, small_object_min_size: int = 50, texture_scale: int = 5, texture_warn: bool = False, benchmark: bool = False, verbose: bool = False)[source]#

Initializes the object with a sequence of operations and measurements for image processing. The sequence includes smoothing, enhance, segmentation, border object removal, and various measurement steps for analyzing images. Customizable parameters allow for adjusting the processing pipeline for specific use cases such as image segmentation and feature extraction.

Parameters:

gaussian_sigma (int) – Standard deviation for Gaussian kernel in smoothing.
gaussian_mode (str) – Mode for handling image boundaries in Gaussian smoothing.
gaussian_truncate (float) – Truncate filter at this many standard deviations.
otsu_ignore_zeros (bool) – Whether to ignore zero pixels in Otsu thresholding.
otsu_ignore_borders (bool) – Whether to ignore border objects in Otsu detection.
mask_opener_footprint (Literal['auto'] | ~numpy.ndarray | int | None) – Structuring element for morphological opening.
border_remover_size (int) – Size of border to remove objects from.
small_object_min_size (int) – Minimum size of objects to retain.
texture_scale (int) – Scale parameter for Haralick texture features.
texture_warn (bool) – Whether to warn on texture computation errors.
shape – Deprecated, use mask_opener_footprint.
min_size – Deprecated, use small_object_min_size.
border_size – Deprecated, use border_remover_size.
benchmark (bool)
verbose (bool)

__copy__() → Self#

Returns a shallow copy of the model.

Return type:: Self

__deepcopy__(memo: dict[int, Any] | None = None) → Self#

Returns a deep copy of the model.

Parameters:: memo (dict[int, Any] | None)
Return type:: Self

__del__()#: Automatically stop tracemalloc when the object is deleted.

classmethod __get_pydantic_json_schema__(core_schema: CoreSchema, handler: GetJsonSchemaHandler, /) → JsonSchemaValue#

Hook into generating the model’s JSON schema.

Parameters:

core_schema (CoreSchema) – A pydantic-core CoreSchema. You can ignore this argument and call the handler with a new CoreSchema, wrap this CoreSchema ({‘type’: ‘nullable’, ‘schema’: current_schema}), or just call the handler with the original schema.
handler (GetJsonSchemaHandler) – Call into Pydantic’s internal JSON schema generation. This will raise a pydantic.errors.PydanticInvalidForJsonSchema if JSON schema generation fails. Since this gets called by BaseModel.model_json_schema you can override the schema_generator argument to that function to change JSON schema generation globally for a type.

Returns:

A JSON schema, as a Python object.

Return type:

JsonSchemaValue

__iter__() → Generator[tuple[str, Any], None, None]#

So dict(model) works.

Return type:: Generator[tuple[str, Any], None, None]

__pretty__(fmt: Callable[[Any], Any], **kwargs: Any) → Generator[Any]#

Used by devtools (https://python-devtools.helpmanual.io/) to pretty print objects.

Parameters:

fmt (Callable[[Any], Any])
kwargs (Any)

Return type:

Generator[Any]

classmethod __pydantic_init_subclass__(**kwargs: Any) → None#

Populate field descriptions from the subclass docstring.

Runs once per concrete subclass after pydantic has built its model. Copies parameter descriptions parsed from the Google-style Args: docstring block onto each field’s description slot so they surface in model_json_schema() — the machine-readable contract used by downstream tooling (e.g. an MCP server).

Parameters:: **kwargs (Any) – Class-keyword arguments forwarded by pydantic.
Return type:: None

classmethod __pydantic_on_complete__() → None#

This is called once the class and its fields are fully initialized and ready to be used.

This typically happens when the class is created (just before [__pydantic_init_subclass__()][pydantic.main.BaseModel.__pydantic_init_subclass__] is called on the superclass), except when forward annotations are used that could not immediately be resolved. In that case, it will be called later, when the model is rebuilt automatically or explicitly using [model_rebuild()][pydantic.main.BaseModel.model_rebuild].

Return type:: None

__repr_name__() → str#

Name of the instance’s class, used in __repr__.

Return type:: str

__repr_recursion__(object: Any) → str#

Returns the string representation of a recursive object.

Parameters:: object (Any)
Return type:: str

__rich_repr__() → RichReprResult#

Used by Rich (https://rich.readthedocs.io/en/stable/pretty.html) to pretty print objects.

Return type:: RichReprResult

__str__() → str#

Return a JSON-formatted string representation of the pipeline.

The generated JSON string provides a structured representation of the object’s current state, including its operations and measurements. This output can be used for logging, debugging, or to recreate the object’s configuration in another context.

Returns:: A JSON-formatted string that encodes the object’s current configuration in a human-readable manner. This includes the phenotypic version, pipeline name, description, and the lists of operations and measurements.
Return type:: str

analyze(df: pandas.DataFrame) → pandas.DataFrame#

Run the analysis chain (filters then model) against an aggregate frame.

Applies each filter in order — each transforms a DataFrame into another DataFrame — then runs the configured terminal model to produce a fit summary (typically one row per group).

The expected input is the post-applied, aggregated DataFrame the CLI seeds into measurements.parquet. analyze does not apply post itself: by default measure() runs self._post before returning, and the CLI applies post explicitly to a copy of the aggregated master before invoking analyze. Callers passing apply_post=False to measure() are responsible for applying post (or accepting that filters/model see pre-post data) before calling analyze.

Parameters:

df (pandas.DataFrame) – The aggregate measurements DataFrame.

Returns:

The model-fit output (one row per group, plus shared: MODEL_METRICS columns for fit quality).

Return type:

pd.DataFrame

Raises:

ValueError – If no model is configured. Configure via set_model() (or pass model= at construction).

apply(image: Image, inplace: bool = False, reset: bool | None = None) → GridImage | Image#

The class provides an interface to process and apply a series of operations on an Image. The operations are maintained in a queue and executed sequentially when applied to the given Image.

Parameters:

image (Image) – The arr Image to be processed. The type Image refers to an instance of the Image object to which transformations are applied.
inplace (bool, optional) – A flag indicating whether to apply the transformations directly on the provided Image (True) or create a copy of the Image before performing transformations (False). Defaults to False.
reset (bool, optional) – Whether to reset the image before applying the pipeline. If None (default), uses the pipeline’s reset setting from __init__. If explicitly set to True or False, overrides the pipeline setting.

Return type:

Union[GridImage, Image]

apply_and_measure(image: Image, inplace: bool = False, reset: bool | None = None, include_metadata: bool = True, apply_post: bool = True) → pd.DataFrame#

Applies processing to the given image and measures the results.

This function first applies a processing method to the supplied image, adjusting it based on the given parameters. After processing, the resulting image is measured, and a DataFrame containing the measurement data is returned.

Parameters:

image (Image) – The image to process and measure.
inplace (bool) – Whether to modify the original image directly or work on a copy. Default is False.
reset (bool, optional) – Whether to reset any previous processing on the image before applying the current method. If None (default), uses the pipeline’s reset setting. If explicitly set, overrides the pipeline setting.
include_metadata (bool) – Whether to include metadata in the measurement results. Default is True.
apply_post (bool) – Forwarded to measure(). When False, the returned DataFrame skips PostMeasurement ops. Defaults to True.

Returns:

A DataFrame containing measurement data for the processed image.

Return type:

pd.DataFrame

apply_napari(image: Image, inplace: bool = False, reset: bool | None = None, viewer: napari.Viewer | None = None) → NapariPipelineResult#

Apply the pipeline and progressively add layers to a napari viewer.

Creates (or reuses) a napari viewer and adds the original image layers as a baseline, then adds the modified layer after each operation completes. Layer names follow the pattern {step:02d}_{OperationName}_{accessor}.

Parameters:

image (Image) – The input image to process.
inplace (bool) – If True the image is modified in place; otherwise a copy is made first. Defaults to False.
reset (bool | None) – Whether to reset the image before applying operations. None (default) uses the pipeline-level setting.
viewer (napari.Viewer | None) – An existing napari viewer to add layers to. If None (default), a new viewer is created.

Returns:

Named tuple with the final image and the napari viewer reference.

Return type:

NapariPipelineResult

Raises:

ImportError – If napari is not installed.

apply_with_intermediates(image: Image, inplace: bool = False, reset: bool | None = None, output_dir: str | Path | None = None, *, full_layers: bool = False) → IntermediateResult#

Apply the pipeline and capture a snapshot of the image after each operation.

Behaves identically to apply() (respecting inplace, reset, benchmark timing, and verbose/tqdm progress) but additionally records the image state after every operation completes.

Parameters:

image (Image) – The input image to process.
inplace (bool) – If True the image is modified in place; otherwise a copy is made first. Defaults to False.
reset (Optional[bool]) – Whether to reset the image before applying operations. None (default) uses the pipeline-level setting.
output_dir (Optional[Union[str, Path]]) – Optional directory path. When provided, each intermediate image is persisted to an HDF5 file inside this directory (created automatically) and the corresponding dict value is set to None to conserve memory. When None, intermediates are kept in memory as Image copies.
full_layers (bool) – When True and output_dir is set, each non-read-only operation’s full image state is written as a complete schema-v2 snapshot via save2hdf5 (all layers + class/grid metadata) instead of the delta layout. Used by the builder’s node-preview cache so any node’s HDF reconstructs a faithful Image/GridImage. Defaults to False.

Returns:

A named tuple containing the final image and a dictionary mapping operation names to intermediate snapshots (or None when output_dir is used).

Return type:

IntermediateResult

benchmark_results() → pandas.DataFrame#

Return execution times and memory usage for operations and measurements.

This method should be called after applying the pipeline on an image to get the execution times and memory consumption of the different processes.

When an operation is itself an ImagePipelineCore (nested pipeline), its sub-operations are expanded as indented sub-rows beneath the parent entry with names like "ParentOp > ChildOp".

Returns:

A DataFrame with columns Process Type,: Process Name, Execution Time (s), Memory Delta (MB), and RSS After (MB).

Return type:

pd.DataFrame

classmethod construct(_fields_set: set[str] | None = None, **values: Any) → Self#

Parameters:

_fields_set (set[str] | None)
values (Any)

Return type:

Self

Returns a copy of the model.

!!! warning “Deprecated”: This method is now deprecated; use model_copy instead.

If you need include or exclude, use:

`python {test="skip" lint="skip"} data = self.model_dump(include=include, exclude=exclude, round_trip=True) data = {**data, **(update or {})} copied = self.model_validate(data) `

Parameters:

include (AbstractSetIntStr | MappingIntStrAny | None) – Optional set or mapping specifying which fields to include in the copied model.
exclude (AbstractSetIntStr | MappingIntStrAny | None) – Optional set or mapping specifying which fields to exclude in the copied model.
update (Dict[str, Any] | None) – Optional dictionary of field-value pairs to override field values in the copied model.
deep (bool) – If True, the values of fields that are Pydantic models will be deep-copied.

Returns:

A copy of the model with included, excluded and updated fields as specified.

Return type:

Self

property desc: str#: Get pipeline description. Returns class docstring if no description set.

Parameters:

include (set[int] | set[str] | Mapping[int, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | Mapping[str, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | None)
exclude (set[int] | set[str] | Mapping[int, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | Mapping[str, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | None)
by_alias (bool)
exclude_unset (bool)
exclude_defaults (bool)
exclude_none (bool)

Return type:

Dict[str, Any]

classmethod from_json(json_data: str | Path | dict, benchmark: bool = False, verbose: bool = False) → PrefabPipeline#

Deserialize a PrefabPipeline from JSON.

PrefabPipeline subclasses build their ops/meas inside __init__, so the base from_json (which passes ops= directly) would conflict. This override deserializes via ImagePipeline and re-tags the instance as the correct PrefabPipeline subclass.

Parameters:

json_data (str | Path | dict) – A JSON string, path to a JSON file, or a pre-parsed dict.
benchmark (bool) – Whether to enable benchmarking for the pipeline.
verbose (bool) – Whether to enable verbose output.

Returns:

A PrefabPipeline (or subclass) instance with the loaded configuration.

Return type:

PrefabPipeline

classmethod from_orm(obj: Any) → Self#

Parameters:: obj (Any)
Return type:: Self

get_filters() → Dict[str, SetAnalyzer]#

Get a copy of the analysis filter chain.

Returns a shallow copy to prevent accidental mutation of internal state.

Returns:

Ordered dict mapping filter names to: SetAnalyzer instances. Empty when no filters configured.

Return type:

Dict[str, SetAnalyzer]

get_meas() → Dict[str, MeasureFeatures]#

Get a copy of the measurements dictionary.

Returns a shallow copy to prevent accidental mutation of internal state.

Returns:

Dictionary mapping measurement names to: MeasureFeatures instances.

Return type:

Dict[str, MeasureFeatures]

get_model() → ModelFitter | None#

Get the analysis endpoint model, if configured.

Returns:

The configured ModelFitter instance,: or None when no model is set.

Return type:

Optional[ModelFitter]

get_ops() → Dict[str, ImageOperation | ImagePipelineCore]#

Get a copy of the operations dictionary.

Returns a shallow copy to prevent accidental mutation of internal state.

Returns:

Dictionary mapping: operation names to ImageOperation instances (or nested pipelines).

Return type:

Dict[str, ImageOperation | ImagePipelineCore]

get_post() → Dict[str, PostMeasurement]#

Get a copy of the post-measurement transforms dictionary.

Returns a shallow copy to prevent accidental mutation of internal state.

Returns:

Dictionary mapping post-measurement: names to PostMeasurement instances.

Return type:

Dict[str, PostMeasurement]

get_qc() → List[QcRecipeEntry]#

Get a copy of the QC config entry list.

Returns a shallow copy so callers cannot mutate the pipeline’s internal list by appending/removing. The entries themselves are shared (they are lightweight config dataclasses); run_qc and the GUI only read them and instantiate fresh checks from their params.

Returns:

Ordered QC config entries. Empty when no: checks are configured.

Return type:

List[QcRecipeEntry]

Parameters:

include (set[int] | set[str] | Mapping[int, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | Mapping[str, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | None)
exclude (set[int] | set[str] | Mapping[int, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | Mapping[str, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | None)
by_alias (bool)
exclude_unset (bool)
exclude_defaults (bool)
exclude_none (bool)
encoder (Callable[[Any], Any] | None)
models_as_dict (bool)
dumps_kwargs (Any)

Return type:

str

measure(image: Image, include_metadata: bool = True, apply_post: bool = True) → pd.DataFrame#

Measures properties of a given image and optionally includes metadata. The method performs measurements using a set of predefined measurement operations. If benchmarking is enabled, the execution time of each measurement is recorded. When verbose mode is active, detailed logging of the measurement process is displayed. A progress bar is used to track progress if the tqdm library is available.

Parameters:

image (Image) – The image object for which measurements are performed. It must support the info method and optionally a grid or objects attribute.
include_metadata (bool, optional) – Indicates whether metadata should be included in the measurements. Defaults to True.
apply_post (bool, optional) – Whether to apply the configured PostMeasurement operations to the merged frame before returning. Defaults to True. Pass False to obtain the pre-post merged DataFrame — useful when the caller (e.g. the CLI) wants to persist a clean copy and apply post separately.

Returns:

A DataFrame containing the results of all performed measurements combined: on the same index. Columns are ordered as [metadata] -> [measurements] -> [MetadataImage_] -> [info block]: user/experimental Metadata* columns first (in canonical REMBI order, present only when include_metadata is True), then the measurement columns, then the framework MetadataImage_* bookkeeping block (per-image provenance: UUID, ImageName, BitDepth, …), then the per-object image-info block (Object_Label followed by the Bbox_* / Grid_* geometry columns) last.

Return type:

pd.DataFrame

Raises:

Exception – An exception is raised if a measurement operation fails while being applied to the image.

model_computed_fields = {}#

model_config: ClassVar[ConfigDict] = {'arbitrary_types_allowed': True, 'extra': 'forbid', 'validate_assignment': True, 'validate_by_name': True}#: Configuration for the model, should be a dictionary conforming to [ConfigDict][pydantic.config.ConfigDict].

classmethod model_construct(_fields_set: set[str] | None = None, **values: Any) → Self#

Creates a new instance of the Model class with validated data.

Creates a new model setting __dict__ and __pydantic_fields_set__ from trusted or pre-validated data. Default values are respected, but no other validation is performed.

!!! note: model_construct() generally respects the model_config.extra setting on the provided model. That is, if model_config.extra == ‘allow’, then all extra passed values are added to the model instance’s __dict__ and __pydantic_extra__ fields. If model_config.extra == ‘ignore’ (the default), then all extra passed values are ignored. Because no validation is performed with a call to model_construct(), having model_config.extra == ‘forbid’ does not result in an error if extra values are passed, but they will be ignored.

Parameters:

_fields_set (set[str] | None) – A set of field names that were originally explicitly set during instantiation. If provided, this is directly used for the [model_fields_set][pydantic.BaseModel.model_fields_set] attribute. Otherwise, the field names from the values argument will be used.
values (Any) – Trusted or pre-validated data dictionary.

Returns:

A new instance of the Model class with validated data.

Return type:

Self

model_copy(*, update: Mapping[str, Any] | None = None, deep: bool = False) → Self#

!!! abstract “Usage Documentation”: [model_copy](../concepts/models.md#model-copy)

Returns a copy of the model.

!!! note: The underlying instance’s [__dict__][object.__dict__] attribute is copied. This might have unexpected side effects if you store anything in it, on top of the model fields (e.g. the value of [cached properties][functools.cached_property]).

Parameters:

update (Mapping[str, Any] | None) – Values to change/add in the new model. Note: the data is not validated before creating the new model. You should trust this data.
deep (bool) – Set to True to make a deep copy of the model.

Returns:

New model instance.

Return type:

Self

!!! abstract “Usage Documentation”: [model_dump](../concepts/serialization.md#python-mode)

Generate a dictionary representation of the model, optionally specifying which fields to include or exclude.

Parameters:

mode (Literal['json', 'python'] | str) – The mode in which to_python should run. If mode is ‘json’, the output will only contain JSON serializable types. If mode is ‘python’, the output may contain non-JSON-serializable Python objects.
include (set[int] | set[str] | Mapping[int, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | Mapping[str, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | None) – A set of fields to include in the output.
exclude (set[int] | set[str] | Mapping[int, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | Mapping[str, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | None) – A set of fields to exclude from the output.
context (Any | None) – Additional context to pass to the serializer.
by_alias (bool | None) – Whether to use the field’s alias in the dictionary key if defined.
exclude_unset (bool) – Whether to exclude fields that have not been explicitly set.
exclude_defaults (bool) – Whether to exclude fields that are set to their default value.
exclude_none (bool) – Whether to exclude fields that have a value of None.
exclude_computed_fields (bool) – Whether to exclude computed fields. While this can be useful for round-tripping, it is usually recommended to use the dedicated round_trip parameter instead.
round_trip (bool) – If True, dumped values should be valid as input for non-idempotent types such as Json[T].
warnings (bool | Literal['none', 'warn', 'error']) – How to handle serialization errors. False/”none” ignores them, True/”warn” logs errors, “error” raises a [PydanticSerializationError][pydantic_core.PydanticSerializationError].
fallback (Callable[[Any], Any] | None) – A function to call when an unknown value is encountered. If not provided, a [PydanticSerializationError][pydantic_core.PydanticSerializationError] error is raised.
serialize_as_any (bool) – Whether to serialize fields with duck-typing serialization behavior.

Returns:

A dictionary representation of the model.

Return type:

dict[str, Any]

!!! abstract “Usage Documentation”: [model_dump_json](../concepts/serialization.md#json-mode)

Generates a JSON representation of the model using Pydantic’s to_json method.

Parameters:

indent (int | None) – Indentation to use in the JSON output. If None is passed, the output will be compact.
ensure_ascii (bool) – If True, the output is guaranteed to have all incoming non-ASCII characters escaped. If False (the default), these characters will be output as-is.
include (set[int] | set[str] | Mapping[int, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | Mapping[str, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | None) – Field(s) to include in the JSON output.
exclude (set[int] | set[str] | Mapping[int, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | Mapping[str, set[int] | set[str] | Mapping[int, IncEx | bool] | Mapping[str, IncEx | bool] | bool] | None) – Field(s) to exclude from the JSON output.
context (Any | None) – Additional context to pass to the serializer.
by_alias (bool | None) – Whether to serialize using field aliases.
exclude_unset (bool) – Whether to exclude fields that have not been explicitly set.
exclude_defaults (bool) – Whether to exclude fields that are set to their default value.
exclude_none (bool) – Whether to exclude fields that have a value of None.
exclude_computed_fields (bool) – Whether to exclude computed fields. While this can be useful for round-tripping, it is usually recommended to use the dedicated round_trip parameter instead.
round_trip (bool) – If True, dumped values should be valid as input for non-idempotent types such as Json[T].
warnings (bool | Literal['none', 'warn', 'error']) – How to handle serialization errors. False/”none” ignores them, True/”warn” logs errors, “error” raises a [PydanticSerializationError][pydantic_core.PydanticSerializationError].
fallback (Callable[[Any], Any] | None) – A function to call when an unknown value is encountered. If not provided, a [PydanticSerializationError][pydantic_core.PydanticSerializationError] error is raised.
serialize_as_any (bool) – Whether to serialize fields with duck-typing serialization behavior.

Returns:

A JSON string representation of the model.

Return type:

str

property model_extra: dict[str, Any] | None#

Get extra fields set during validation.

Returns:: A dictionary of extra fields, or None if config.extra is not set to “allow”.

model_fields = {'benchmark': FieldInfo(annotation=bool, required=False, default=False, description='Whether to enable execution time tracking for operations and measurements.'), 'desc_value': FieldInfo(annotation=Union[str, NoneType], required=False, default=None, alias='desc', alias_priority=2), 'filters': FieldInfo(annotation=Dict[str, SetAnalyzer], required=False, default={}), 'meas': FieldInfo(annotation=Dict[str, MeasureFeatures], required=False, default={}), 'model': FieldInfo(annotation=Union[ModelFitter, NoneType], required=False, default=None), 'name': FieldInfo(annotation=str, required=False, default_factory=<lambda>, description='A unique identifier for this pipeline. Defaults to a randomly generated UUID4 string if not provided during initialization.'), 'ncols': FieldInfo(annotation=Union[int, NoneType], required=False, default=None), 'nrows': FieldInfo(annotation=Union[int, NoneType], required=False, default=None), 'ops': FieldInfo(annotation=Dict[str, Union[ImageOperation, ImagePipelineCore]], required=False, default_factory=dict, alias_priority=2, validation_alias=AliasChoices(choices=['ops', 'pipe_cfgs'])), 'post': FieldInfo(annotation=Dict[str, PostMeasurement], required=False, default={}), 'qc': FieldInfo(annotation=List[QcRecipeEntry], required=False, default_factory=list, exclude=True), 'reset': FieldInfo(annotation=bool, required=False, default=False), 'verbose': FieldInfo(annotation=bool, required=False, default=False, description='Whether to enable verbose logging during pipeline execution.')}#

property model_fields_set: set[str]#

Returns the set of fields that have been explicitly set on this model instance.

Returns:

A set of strings representing the fields that have been set,: i.e. that were not filled from defaults.

classmethod model_json_schema(by_alias: bool = True, ref_template: str = '#/$defs/{model}', schema_generator: type[~pydantic.json_schema.GenerateJsonSchema] = <class 'pydantic.json_schema.GenerateJsonSchema'>, mode: ~typing.Literal['validation', 'serialization'] = 'validation', *, union_format: ~typing.Literal['any_of', 'primitive_type_array'] = 'any_of') → dict[str, Any]#

Generates a JSON schema for a model class.

Parameters:

by_alias (bool) – Whether to use attribute aliases or not.
ref_template (str) – The reference template.
union_format (Literal['any_of', 'primitive_type_array']) –
The format to use when combining schemas from unions together. Can be one of:
- ’any_of’: Use the [anyOf](https://json-schema.org/understanding-json-schema/reference/combining#anyOf)
keyword to combine schemas (the default). - ‘primitive_type_array’: Use the [type](https://json-schema.org/understanding-json-schema/reference/type) keyword as an array of strings, containing each type of the combination. If any of the schemas is not a primitive type (string, boolean, null, integer or number) or contains constraints/metadata, falls back to any_of.
schema_generator (type[GenerateJsonSchema]) – To override the logic used to generate the JSON schema, as a subclass of GenerateJsonSchema with your desired modifications
mode (Literal['validation', 'serialization']) – The mode in which to generate the schema.

Returns:

The JSON schema for the given model class.

Return type:

dict[str, Any]

classmethod model_parametrized_name(params: tuple[type[Any], ...]) → str#

Compute the class name for parametrizations of generic classes.

This method can be overridden to achieve a custom naming scheme for generic BaseModels.

Parameters:: params (tuple[type[Any], ...]) – Tuple of types of the class. Given a generic class Model with 2 type variables and a concrete model Model[str, int], the value (str, int) would be passed to params.
Returns:: String representing the new class where params are passed to cls as type variables.
Raises:: TypeError – Raised when trying to generate concrete names for non-generic models.
Return type:: str

model_post_init(_BaseOperation__context: Any) → None#

Initialize logging and memory tracking after model construction.

Replaces the legacy __init__ body: creates the per-class logger and, when that logger is enabled for INFO level or higher, starts tracemalloc so per-operation memory usage can be logged.

Parameters:

__context – Pydantic post-init context (unused).
_BaseOperation__context (Any)

Return type:

None

classmethod model_rebuild(*, force: bool = False, raise_errors: bool = True, _parent_namespace_depth: int = 2, _types_namespace: MappingNamespace | None = None) → bool | None#

Try to rebuild the pydantic-core schema for the model.

This may be necessary when one of the annotations is a ForwardRef which could not be resolved during the initial attempt to build the schema, and automatic rebuilding fails.

Parameters:

force (bool) – Whether to force the rebuilding of the model schema, defaults to False.
raise_errors (bool) – Whether to raise errors, defaults to True.
_parent_namespace_depth (int) – The depth level of the parent namespace, defaults to 2.
_types_namespace (MappingNamespace | None) – The types namespace, defaults to None.

Returns:

Returns None if the schema is already “complete” and rebuilding was not required. If rebuilding _was_ required, returns True if rebuilding was successful, otherwise False.

Return type:

bool | None

Validate a pydantic model instance.

Parameters:

obj (Any) – The object to validate.
strict (bool | None) – Whether to enforce types strictly.
extra (Literal['allow', 'ignore', 'forbid'] | None) – Whether to ignore, allow, or forbid extra data during model validation. See the [extra configuration value][pydantic.ConfigDict.extra] for details.
from_attributes (bool | None) – Whether to extract data from object attributes.
context (Any | None) – Additional context to pass to the validator.
by_alias (bool | None) – Whether to use the field’s alias when validating against the provided input data.
by_name (bool | None) – Whether to use the field’s name when validating against the provided input data.

Raises:

ValidationError – If the object could not be validated.

Returns:

The validated model instance.

Return type:

Self

!!! abstract “Usage Documentation”: [JSON Parsing](../concepts/json.md#json-parsing)

Validate the given JSON data against the Pydantic model.

Parameters:

json_data (str | bytes | bytearray) – The JSON data to validate.
strict (bool | None) – Whether to enforce types strictly.
extra (Literal['allow', 'ignore', 'forbid'] | None) – Whether to ignore, allow, or forbid extra data during model validation. See the [extra configuration value][pydantic.ConfigDict.extra] for details.
context (Any | None) – Extra variables to pass to the validator.
by_alias (bool | None) – Whether to use the field’s alias when validating against the provided input data.
by_name (bool | None) – Whether to use the field’s name when validating against the provided input data.

Returns:

The validated Pydantic model.

Raises:

ValidationError – If json_data is not a JSON string or the object could not be validated.

Return type:

Self

Validate the given object with string data against the Pydantic model.

Parameters:

obj (Any) – The object containing string data to validate.
strict (bool | None) – Whether to enforce types strictly.
extra (Literal['allow', 'ignore', 'forbid'] | None) – Whether to ignore, allow, or forbid extra data during model validation. See the [extra configuration value][pydantic.ConfigDict.extra] for details.
context (Any | None) – Extra variables to pass to the validator.
by_alias (bool | None) – Whether to use the field’s alias when validating against the provided input data.
by_name (bool | None) – Whether to use the field’s name when validating against the provided input data.

Returns:

The validated Pydantic model.

Return type:

Self

classmethod parse_file(path: str | Path, *, content_type: str | None = None, encoding: str = 'utf8', proto: DeprecatedParseProtocol | None = None, allow_pickle: bool = False) → Self#

Parameters:

path (str | Path)
content_type (str | None)
encoding (str)
proto (DeprecatedParseProtocol | None)
allow_pickle (bool)

Return type:

Self

classmethod parse_obj(obj: Any) → Self#

Parameters:: obj (Any)
Return type:: Self

classmethod parse_raw(b: str | bytes, *, content_type: str | None = None, encoding: str = 'utf8', proto: DeprecatedParseProtocol | None = None, allow_pickle: bool = False) → Self#

Parameters:

b (str | bytes)
content_type (str | None)
encoding (str)
proto (DeprecatedParseProtocol | None)
allow_pickle (bool)

Return type:

Self

classmethod schema(by_alias: bool = True, ref_template: str = '#/$defs/{model}') → Dict[str, Any]#

Parameters:

by_alias (bool)
ref_template (str)

Return type:

Dict[str, Any]

classmethod schema_json(*, by_alias: bool = True, ref_template: str = '#/$defs/{model}', **dumps_kwargs: Any) → str#

Parameters:

by_alias (bool)
ref_template (str)
dumps_kwargs (Any)

Return type:

str

set_filters(filters: List[SetAnalyzer] | Dict[str, SetAnalyzer]) → None#

Set the analysis filter chain.

Parameters:: filters (List[SetAnalyzer] | Dict[str, SetAnalyzer]) – A list or dictionary of SetAnalyzer instances. If a list, class names (deduplicated by suffix) are used as keys.
Raises:: TypeError – If filters is neither a list nor a dictionary.
Return type:: None

set_meas(measurements: List[MeasureFeatures] | Dict[str, MeasureFeatures])#

Sets the measurements to be used for further computation. The input can be either a list of MeasureFeatures objects or a dictionary with string keys and MeasureFeatures objects as values.

The method processes the given input to construct a dictionary mapping measurement names to MeasureFeatures instances. If a list is passed, unique class names of the MeasureFeatures instances in the list are used as keys.

Parameters:: measurements (List[MeasureFeatures] | Dict[str, MeasureFeatures]) – A collection of measurement features either as a list of MeasureFeatures objects, where class names are used as keys for dictionary creation, or as a dictionary where keys are predefined strings and values are MeasureFeatures objects.
Raises:: TypeError – If the measurements argument is neither a list nor a dictionary.

set_model(model: ModelFitter | None) → None#

Set the analysis endpoint model.

Only one model can be configured per pipeline; assigning a new value replaces any prior model. Pass None to clear.

Parameters:: model (ModelFitter | None) – A ModelFitter instance, or None to clear.
Raises:: TypeError – If model is not a ModelFitter instance and not None.
Return type:: None

set_ops(ops: List[ImageOperation | ImagePipeline] | Dict[str, ImageOperation | ImagePipeline])#

Sets the operations to be performed. The operations can be passed as either a list of ImageOperation or ImagePipeline instances or a dictionary mapping operation names to ImageOperation or ImagePipeline instances. This method ensures that each operation in the list has a unique name. Raises a TypeError if the input is neither a list nor a dictionary.

Parameters:: ops (List[ImageOperation | ImagePipeline] | Dict[str, ImageOperation | ImagePipeline]) – A list of ImageOperation or ImagePipeline objects, or a dictionary where keys are operation names and values are ImageOperation or ImagePipeline objects.
Raises:: TypeError – If the input is not a list or a dictionary.

set_post(post: List[PostMeasurement] | Dict[str, PostMeasurement])#

Set the post-measurement transforms.

Parameters:: post (List[PostMeasurement] | Dict[str, PostMeasurement]) – A list or dictionary of PostMeasurement objects. If a list, class names are used as keys.
Raises:: TypeError – If post is neither a list nor a dictionary.

set_qc(qc: List[QcRecipeEntry] | None) → None#

Set the QC config entry list.

Mirrors set_post() / set_filters() but the QC section is an ordered list of QcRecipeEntry (carrying stable instance_id/enabled metadata), not a name-keyed dict.

Parameters:: qc (List[QcRecipeEntry] | None) – A list of QcRecipeEntry, or None to clear.
Raises:: TypeError – If qc is neither a list nor None (raised by the _normalize_qc validator on assignment).
Return type:: None

to_json(filepath: str | Path | None = None) → str | None#

Serialize the pipeline configuration to JSON format.

This method captures the pipeline’s operations and measurements. It excludes internal state (pydantic PrivateAttr fields) and pandas DataFrames to keep the serialization clean and focused on reproducible configuration.

Parameters:: filepath (str | Path | None) – Optional path to save the JSON. If None, returns JSON string. Can be a string or Path object.
Returns:: JSON string representation of the pipeline configuration.
Return type:: str

Example

Serialize a pipeline to JSON format:

>>> from phenotypic import ImagePipeline
>>> from phenotypic.detect import OtsuDetector
>>> from phenotypic.measure import MeasureShape
>>> pipe = ImagePipeline(pipe_cfgs=[OtsuDetector()], meas=[MeasureShape()])
>>> json_str = pipe.to_json()
>>> pipe.to_json('my_pipeline')  # Save to typed config file

classmethod update_forward_refs(**localns: Any) → None#

Parameters:: localns (Any)
Return type:: None

classmethod validate(value: Any) → Self#

Parameters:: value (Any)
Return type:: Self

widget(image: Image | None = None, show: bool = False) → Widget#

Return (and optionally display) the root widget.

Parameters:

image (Image | None) – Optional image to visualize. If provided, visualization controls will be added to the widget.
show (bool) – Whether to display the widget immediately. Defaults to False.

Returns:

The root widget.

Return type:

ipywidgets.Widget

Raises:

ImportError – If ipywidgets or IPython are not installed.

name: str#

desc_value: str | None#

benchmark: bool#

verbose: bool#

reset: bool#

nrows: int | None#

ncols: int | None#

ops: Dict[str, ImageOperation | 'ImagePipelineCore']#

meas: Dict[str, MeasureFeatures]#

post: Dict[str, PostMeasurement]#

filters: Dict[str, SetAnalyzer]#

model: ModelFitter | None#

qc: List[QcRecipeEntry]#

phenotypic.prefab.HeavyOtsuPipeline#

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