Source code for phenotypic.refine._border_object_modifier
from __future__ import annotations
from typing import TYPE_CHECKING
if TYPE_CHECKING:
from phenotypic._core._image import Image
import numpy as np
from typing import Optional, Union
from phenotypic.abc_ import ObjectRefiner
[docs]
class BorderObjectRemover(ObjectRefiner):
"""Remove colonies that touch the image border within a configurable margin.
Zeroes any labeled objects in ``objmap`` whose pixels fall within a
border band, ensuring only fully contained colonies are analyzed.
Partial edge colonies bias size and shape measurements.
Args:
border_size: Width of the exclusion border. ``None`` uses 1% of
the smaller dimension. Float in (0, 1) is a fraction of the
image size. Int or float >= 1 is absolute pixels. Default: 1.
Returns:
Image: Input image with ``objmask`` and ``objmap`` updated to
exclude border-touching objects.
Raises:
TypeError: If ``border_size`` type is invalid.
Best For:
- Plates where the plate rim or image crop truncates edge colonies.
- Grid assays where border wells are partially visible.
- Automated crops that shift between frames.
Consider Also:
- :class:`SmallObjectRemover` for removing noise fragments that are
not necessarily at the border.
- :class:`GridOversizedObjectRemover` for removing abnormally large
objects that span multiple grid sections.
See Also:
:doc:`/how_to/notebooks/refine_noisy_boundaries` for a walkthrough
of refinement operations.
:doc:`/explanation/refinement_strategies` for choosing the right
refinement sequence.
"""
[docs]
def __init__(self, border_size: Optional[Union[int, float]] = 1):
"""Initialize the remover.
Args:
border_size: Width of the exclusion border around the image.
- ``None``: Use a default margin equal to 1% of the smaller
image dimension.
- ``float`` in (0, 1): Interpret as a fraction of the minimum
image dimension, producing a resolution-adaptive margin.
- ``int`` or ``float`` ≥ 1: Interpret as an absolute number of
pixels.
Notes:
Larger margins remove more edge-touching colonies and are useful
when crops are loose or the plate rim intrudes. Smaller margins
preserve edge colonies but risk including partial objects.
"""
self.border_size = border_size
def _operate(self, image: Image) -> Image:
if self.border_size is None:
edge_size = int(np.min(image.shape[[1, 2]]) * 0.01)
elif type(self.border_size) == float and 0.0 < self.border_size < 1.0:
edge_size = int(np.min(image.shape) * self.border_size)
elif isinstance(self.border_size, (int, float)):
edge_size = self.border_size
else:
raise TypeError(
"Invalid edge size. Should be int, float, or None to use default edge size."
)
obj_map = image.objmap[:]
edges = [
obj_map[: edge_size - 1, :].ravel(),
obj_map[-edge_size:, :].ravel(),
obj_map[:, : edge_size - 1].ravel(),
obj_map[:, -edge_size:].ravel(),
]
edge_labels = np.unique(np.concatenate(edges))
for label in edge_labels:
obj_map[obj_map == label] = 0
image.objmap = obj_map
return image