Source code for phenotypic.detect._mean_detector
from __future__ import annotations
from typing import TYPE_CHECKING
if TYPE_CHECKING:
from phenotypic._core._image import Image
from skimage.filters import threshold_mean
from skimage.segmentation import clear_border
from ..abc_ import ThresholdDetector
[docs]
class MeanDetector(ThresholdDetector):
"""Detect colonies by thresholding at the mean image intensity.
Use the arithmetic mean of all pixel intensities as the threshold,
classifying pixels above the mean as colony foreground. This
parameter-free baseline is fast and deterministic, making it useful for
quick sanity checks or as a fallback when histogram-adaptive methods
produce unexpected results. For a full comparison see
:doc:`/explanation/detection_strategies_compared`.
Args:
ignore_zeros: Exclude zero-intensity pixels from threshold
computation. Enable for plates with black borders or masked
regions; disable only when zero is a meaningful intensity value.
Default: True.
ignore_borders: Remove colonies touching image edges via
``clear_border()``. Recommended for grid-based colony counting
to eliminate partial colonies at plate boundaries. Default: True.
Returns:
Image: Input image with ``objmask`` set to binary mask and
``objmap`` set to labeled connected components.
Raises:
ValueError: If threshold computation fails (e.g., all pixels share
the same intensity value).
Best For:
* Quick baseline detection requiring no parameter tuning.
* Sanity-checking preprocessing steps before applying a more
specialised detector.
* Plates where colony and background areas are roughly equal in
size.
* Debugging pipelines where a simple, predictable threshold is
needed.
Consider Also:
* :class:`OtsuDetector` for a statistically optimal threshold that
adapts to the histogram shape.
* :class:`TriangleDetector` when colonies are sparse and the
histogram is strongly skewed toward background.
* :class:`IsodataDetector` for an iterative refinement that
converges beyond the simple mean.
See Also:
:doc:`/tutorials/notebooks/02_detecting_colonies`
Step-by-step tutorial for basic colony detection.
:doc:`/how_to/notebooks/choose_detection_algorithm`
Guide for selecting the right detector for your plate images.
:doc:`/explanation/detection_strategies_compared`
In-depth comparison of all detection strategies.
"""
def __init__(self, ignore_zeros: bool = False, ignore_borders: bool = True):
self.ignore_zeros = ignore_zeros
self.ignore_borders = ignore_borders
def _operate(self, image: Image) -> Image:
"""Binarizes the given image matrix using the Mean threshold method.
This function modifies the arr image by applying a binary mask to
its enhanced matrix (`detect_mat`). The binarization threshold is
automatically determined using Mean method. The resulting binary
mask is stored in the image's `objmask` attribute.
Args:
image (Image): The arr image object. It must have an `detect_mat`
attribute, which is used as the basis for creating the binary mask.
Returns:
Image: The arr image object with its `objmask` attribute updated
to the computed binary mask other_image.
"""
enh_matrix = image.detect_mat[:]
mask = image.detect_mat[:] >= threshold_mean(
enh_matrix[enh_matrix != 0] if self.ignore_zeros else enh_matrix
)
mask = clear_border(mask) if self.ignore_borders else mask
image.objmask = mask
return image
# Set the docstring so that it appears in the sphinx documentation
MeanDetector.apply.__doc__ = MeanDetector._operate.__doc__