Source code for phenotypic.detect._li_detector
from __future__ import annotations
from typing import TYPE_CHECKING
if TYPE_CHECKING:
from phenotypic._core._image import Image
from skimage.filters import threshold_li
from skimage.segmentation import clear_border
from ..abc_ import ThresholdDetector
[docs]
class LiDetector(ThresholdDetector):
"""Detect colonies by minimising cross-entropy between the original and thresholded image.
Iteratively refine a threshold that minimises the information loss
(cross-entropy) between the original intensity distribution and the
binarised result. Performs well on low-contrast or noisy plates where
the histogram is not clearly bimodal and Otsu's variance-based
assumption does not hold. For a full comparison see
:doc:`/explanation/detection_strategies_compared`.
Args:
ignore_zeros: Exclude zero-intensity pixels from threshold
computation. Enable for plates with black borders or masked
regions; disable only when zero is a meaningful intensity value.
Default: True.
ignore_borders: Remove colonies touching image edges via
``clear_border()``. Recommended for grid-based colony counting
to eliminate partial colonies at plate boundaries. Default: True.
Returns:
Image: Input image with ``objmask`` set to binary mask and
``objmap`` set to labeled connected components.
Raises:
ValueError: If threshold computation fails (e.g., degenerate
histogram with insufficient intensity variation).
Best For:
* Low-contrast plates where colony and background intensities
overlap significantly.
* Noisy or textured agar backgrounds that create histogram
irregularities breaking bimodal assumptions.
* Images where the intensity distribution is unimodal or only
weakly bimodal.
Consider Also:
* :class:`OtsuDetector` when the histogram is clearly bimodal and
a fast single-pass threshold suffices.
* :class:`YenDetector` for a correlation-based alternative that
handles skewed histograms.
* :class:`HysteresisDetector` when colony brightness varies across
the plate and a single threshold under-segments faint regions.
References:
[1] C. H. Li and C. K. Lee, "Minimum cross entropy thresholding,"
*Pattern Recognit.*, vol. 26, no. 4, pp. 617--625, 1993.
See Also:
:doc:`/tutorials/notebooks/02_detecting_colonies`
Step-by-step tutorial for basic colony detection.
:doc:`/how_to/notebooks/choose_detection_algorithm`
Guide for selecting the right detector for your plate images.
:doc:`/explanation/detection_strategies_compared`
In-depth comparison of all detection strategies.
"""
def __init__(self, ignore_zeros: bool = False, ignore_borders: bool = True):
self.ignore_zeros = ignore_zeros
self.ignore_borders = ignore_borders
def _operate(self, image: Image) -> Image:
"""Binarizes the given image matrix using Li's threshold method.
This function modifies the arr image by applying a binary mask to
its enhanced matrix (`detect_mat`). The binarization threshold is
automatically determined using Li's iterative Minimum Cross Entropy method.
The resulting binary mask is stored in the image's `objmask` attribute.
Args:
image (Image): The arr image object. It must have an `detect_mat`
attribute, which is used as the basis for creating the binary mask.
Returns:
Image: The arr image object with its `objmask` attribute updated
to the computed binary mask other_image.
"""
enh_matrix = image.detect_mat[:]
mask = image.detect_mat[:] >= threshold_li(
enh_matrix[enh_matrix != 0] if self.ignore_zeros else enh_matrix
)
mask = clear_border(mask) if self.ignore_borders else mask
image.objmask = mask
return image
# Set the docstring so that it appears in the sphinx documentation
LiDetector.apply.__doc__ = LiDetector._operate.__doc__