Source code for phenotypic.detect._li_detector

from __future__ import annotations

from typing import TYPE_CHECKING

if TYPE_CHECKING:
    from phenotypic._core._image import Image
from skimage.filters import threshold_li
from skimage.segmentation import clear_border

from ..abc_ import ThresholdDetector


[docs] class LiDetector(ThresholdDetector): """Detect colonies by minimising cross-entropy between the original and thresholded image. Iteratively refine a threshold that minimises the information loss (cross-entropy) between the original intensity distribution and its binarised form. Performs well on low-contrast or noisy plates where the histogram is not clearly bimodal and variance-based assumptions may not hold. For a full comparison of detection strategies see :doc:`/explanation/detection_strategies_compared`. Best For: - Low-contrast plates where colony and background intensities overlap significantly. - Noisy or textured agar backgrounds that create histogram irregularities breaking bimodal assumptions. - Images where the intensity distribution is unimodal or only weakly bimodal. Consider Also: - :class:`OtsuDetector` when the histogram is clearly bimodal and a single-pass variance-minimising threshold suffices. - :class:`YenDetector` for a correlation-based alternative that handles skewed histograms. - :class:`HysteresisDetector` when colony brightness varies across the plate and a single threshold under-segments faint regions. Args: ignore_zeros: Exclude zero-intensity pixels from the histogram before computing the threshold. Enable for plates with black borders or masked regions. Default: False. ignore_borders: Remove colonies touching image edges via ``clear_border()``. Recommended for grid-based colony counting to eliminate partial colonies at plate boundaries. Default: True. Returns: Image: Input image with ``objmask`` set to the binary colony mask and ``objmap`` set to labeled connected components. Raises: ValueError: If threshold computation fails due to a degenerate histogram with insufficient intensity variation. References: [1] C. H. Li and C. K. Lee, "Minimum cross entropy thresholding," *Pattern Recognit.*, vol. 26, no. 4, pp. 617--625, 1993. See Also: :doc:`/tutorials/notebooks/02_detecting_colonies` for a step-by-step tutorial on basic colony detection. :doc:`/how_to/notebooks/choose_detection_algorithm` for guidance on selecting the right detector for your plate images. :doc:`/explanation/detection_strategies_compared` for an in-depth comparison of all thresholding strategies. """ ignore_zeros: bool = False ignore_borders: bool = True def _operate(self, image: Image) -> Image: """Binarizes the given image matrix using Li's threshold method. This function modifies the arr image by applying a binary mask to its enhanced matrix (`detect_mat`). The binarization threshold is automatically determined using Li's iterative Minimum Cross Entropy method. The resulting binary mask is stored in the image's `objmask` attribute. Args: image (Image): The arr image object. It must have an `detect_mat` attribute, which is used as the basis for creating the binary mask. Returns: Image: The arr image object with its `objmask` attribute updated to the computed binary mask other_image. """ enh_matrix = image.detect_mat[:] mask = image.detect_mat[:] >= threshold_li( enh_matrix[enh_matrix != 0] if self.ignore_zeros else enh_matrix ) mask = clear_border(mask) if self.ignore_borders else mask image.objmask = mask return image
# Set the docstring so that it appears in the sphinx documentation LiDetector.apply.__doc__ = LiDetector._operate.__doc__