from __future__ import annotations
import math
from typing import Iterable, Tuple
import numpy as np
import phenotypic
from phenotypic.data._sample_image_data import __current_file_dir
# --- Global Helper Functions ---
def _perlin_noise(
h: int, w: int, scales: Iterable[int], rng: np.random.Generator
) -> np.ndarray:
"""
Generates normalized perlin-like noise in [0, 1].
Refactored from _perlin_like to be a standalone helper.
"""
acc = np.zeros((h, w), dtype=np.float32)
total = 0.0
for s in scales:
gh, gw = max(1, h // s), max(1, w // s)
g = rng.random((gh + 1, gw + 1)).astype(np.float32)
y = np.linspace(0, gh, h, endpoint=False)
x = np.linspace(0, gw, w, endpoint=False)
y0 = np.floor(y).astype(int)
x0 = np.floor(x).astype(int)
y1 = np.clip(y0 + 1, 0, gh)
x1 = np.clip(x0 + 1, 0, gw)
wy = y - y0
wx = x - x0
a = g[y0[:, None], x0[None, :]]
b = g[y0[:, None], x1[None, :]]
c = g[y1[:, None], x0[None, :]]
d = g[y1[:, None], x1[None, :]]
acc += (a * (1 - wx) + b * wx) * (1 - wy)[:, None] + (
c * (1 - wx) + d * wx
) * wy[:, None]
total += 1.0
acc = acc / max(total, 1e-6)
return (acc - acc.min()) / (np.ptp(acc) + 1e-6)
def _radial_colony_mask(
h: int, w: int, cy: float, cx: float, base_r: float, rng: np.random.Generator
) -> np.ndarray:
"""Generates the soft, textured mask for the solid colony core."""
yy, xx = np.mgrid[0:h, 0:w]
# Polar coordinates
dy, dx = yy - cy, xx - cx
d = np.sqrt(dy ** 2 + dx ** 2)
theta = np.arctan2(dy, dx)
# Radial noise (irregular radius)
ntheta = 512
ang = np.linspace(-math.pi, math.pi, ntheta, endpoint=False)
radial_noise = 0.08 * rng.standard_normal(ntheta).astype(np.float32)
r_lookup = base_r * (
1.0 + np.interp(theta, ang, radial_noise, period=2 * math.pi)
)
# Soft edge thresholding
edge_soft = max(base_r * 0.05, 1.0)
t = (r_lookup - d) / edge_soft
mask = np.clip(0.5 * (np.tanh(t) + 1.0), 0.0, 1.0)
# Internal texture
tex = _perlin_noise(h, w, scales=(32, 16, 8), rng=rng)
return np.clip(mask * (0.85 + 0.15 * tex), 0.0, 1.0)
def _filament_mask(
h: int,
w: int,
cy: float,
cx: float,
base_r: float,
density: float,
reach_factor: float,
rng: np.random.Generator,
) -> np.ndarray:
"""
Generates a mask for filamentous hyphae with anti-aliasing and solid connections.
"""
# 1. Define Bounding Box
max_reach = base_r * reach_factor
pad = 10
y_min = max(0, int(cy - max_reach - pad))
y_max = min(h, int(cy + max_reach + pad))
x_min = max(0, int(cx - max_reach - pad))
x_max = min(w, int(cx + max_reach + pad))
sh, sw = y_max - y_min, x_max - x_min
if sh <= 0 or sw <= 0:
return np.zeros((h, w), dtype=np.float32)
# 2. Local Grid
yy, xx = np.mgrid[y_min:y_max, x_min:x_max]
dy, dx = yy - cy, xx - cx
dist = np.sqrt(dy ** 2 + dx ** 2)
dist = np.maximum(dist, 1e-5) # Avoid div by zero
theta = np.arctan2(dy, dx)
# 3. Domain Warping (Large scales = gentle curves, no jagged static)
noise = _perlin_noise(sh, sw, scales=(int(sh / 1.5), int(sh / 3)), rng=rng)
warp_strength = np.clip((dist - base_r * 0.5) / base_r, 0.0, 1.0)
warped_theta = theta + (noise - 0.5) * 0.5 * warp_strength
# 4. Anti-Aliased Density
# Limit max frequency by circumference to prevent dots/aliasing
max_freq = (2 * np.pi * dist) / 3.0
safe_density = np.minimum(density, max_freq)
# 5. Continuous Waveform (Connected Branches)
signal = np.cos(warped_theta * safe_density)
# Threshold to create strands
strand_width = 0.8
strands = np.clip((signal - strand_width) * 5.0, 0.0, 1.0)
# 6. Radial Masking (Hard Fade)
d_norm = (dist - base_r * 0.5) / (max_reach - base_r * 0.5)
# Power of 2 cleans up fringe noise
radial_fade = np.clip(1.0 - d_norm, 0.0, 1.0) ** 2.0
# Soft connection to core
core_blend = np.clip((dist - base_r * 0.6) / (base_r * 0.2), 0.0, 1.0)
mask_slice = strands * radial_fade * core_blend
# Texture
mask_slice *= (0.8 + 0.2 * noise)
full_mask = np.zeros((h, w), dtype=np.float32)
full_mask[y_min:y_max, x_min:x_max] = mask_slice
return full_mask
def _create_agar_background(
h: int, w: int, agar_rgb: Tuple[float, float, float], rng: np.random.Generator
) -> np.ndarray:
"""Creates the textured agar background."""
agar = np.array(agar_rgb, dtype=np.float32)
# Scaled noise for texture
bg_noise = _perlin_noise(h, w, scales=(128, 64, 32), rng=rng)
bg_tex = 0.025 * (bg_noise - 0.5)
return np.clip(agar[None, None, :] + bg_tex[..., None], 0.0, 1.0)
def _screen_blend(
bg_img: np.ndarray, mask: np.ndarray, colony_rgb: np.ndarray
) -> np.ndarray:
"""Helper for screen blending colonies (ensures they are lighter than bg)."""
col = np.clip(colony_rgb, 0.86, 0.99)
if col.ndim == 1:
col = col[None, None, :]
colony_region = 1.0 - (1.0 - bg_img) * (1.0 - col)
return bg_img * (1.0 - mask) + colony_region * mask
def _quantize(img: np.ndarray, bit_depth: int) -> np.ndarray:
"""Converts float [0,1] image to uint8/uint16."""
img = np.clip(img, 0.0, 1.0)
if bit_depth == 8:
return (img * 255.0 + 0.5).astype(np.uint8)
elif bit_depth == 16:
return (img * 65535.0 + 0.5).astype(np.uint16)
else:
raise ValueError("bit_depth must be 8 or 16")
# --- Main Generators ---
[docs]
def make_synthetic_colony(
h: int = 256,
w: int = 256,
bit_depth: int = 8,
colony_rgb: Tuple[float, float, float] = (0.96, 0.88, 0.82),
agar_rgb: Tuple[float, float, float] = (0.55, 0.56, 0.54),
seed: int = 1,
) -> np.ndarray:
"""Generate a single bright fungal colony on solid-media agar."""
rng = np.random.default_rng(seed)
# 1. Background
img = _create_agar_background(h, w, agar_rgb, rng)
# 2. Geometry
cy, cx = h * 0.5, w * 0.5
r = min(h, w) * 0.35
# 3. Mask
m = _radial_colony_mask(h, w, cy, cx, r, rng)[..., None]
# 4. Blend
col_rgb = np.array(colony_rgb, dtype=np.float32)
img = _screen_blend(img, m, col_rgb)
return _quantize(img, bit_depth)
[docs]
def make_synthetic_plate(
nrows: int = 8,
ncols: int = 12,
plate_h: int = 2048,
plate_w: int = 3072,
bit_depth: int = 8,
colony_rgb: Tuple[float, float, float] = (0.96, 0.88, 0.82),
agar_rgb: Tuple[float, float, float] = (0.55, 0.56, 0.54),
seed: int = 1,
spacing_factor: float = 0.85,
colony_size_variation: float = 0.15,
) -> np.ndarray:
"""Generate a synthetic array plate with multiple circular colonies."""
rng = np.random.default_rng(seed)
# 1. Background
img = _create_agar_background(plate_h, plate_w, agar_rgb, rng)
# 2. Grid Setup
margin_y = plate_h / (nrows + 1)
margin_x = plate_w / (ncols + 1)
spacing_y = plate_h / (nrows + 1)
spacing_x = plate_w / (ncols + 1)
base_r = min(spacing_y, spacing_x) * spacing_factor * 0.5
col_rgb = np.array(colony_rgb, dtype=np.float32)
# 3. Iterate
for row in range(nrows):
for col_idx in range(ncols):
cy = margin_y + row * spacing_y + rng.uniform(-spacing_y * 0.05,
spacing_y * 0.05)
cx = margin_x + col_idx * spacing_x + rng.uniform(-spacing_x * 0.05,
spacing_x * 0.05)
r = base_r * (1.0 + rng.uniform(-colony_size_variation,
colony_size_variation))
m = _radial_colony_mask(plate_h, plate_w, cy, cx, r, rng)[..., None]
img = _screen_blend(img, m, col_rgb)
return _quantize(img, bit_depth)
[docs]
def make_synthetic_filamentous_plate(
nrows: int = 8,
ncols: int = 12,
plate_h: int = 2048,
plate_w: int = 3072,
bit_depth: int = 8,
colony_rgb: Tuple[float, float, float] = (0.96, 0.90, 0.85),
agar_rgb: Tuple[float, float, float] = (0.55, 0.56, 0.54),
seed: int = 1,
spacing_factor: float = 0.85,
colony_size_variation: float = 0.15,
filament_density: float = 100.0,
filament_reach: float = 2.2,
) -> np.ndarray:
"""
Generate a synthetic plate with filamentous (hairy/branching) colonies.
"""
rng = np.random.default_rng(seed)
# 1. Background
img = _create_agar_background(plate_h, plate_w, agar_rgb, rng)
# 2. Grid Setup
margin_y = plate_h / (nrows + 1)
margin_x = plate_w / (ncols + 1)
spacing_y = plate_h / (nrows + 1)
spacing_x = plate_w / (ncols + 1)
base_r_global = min(spacing_y, spacing_x) * spacing_factor * 0.5
col_rgb = np.array(colony_rgb, dtype=np.float32)
# 3. Iterate
for row in range(nrows):
for col_idx in range(ncols):
cy = margin_y + row * spacing_y + rng.uniform(-spacing_y * 0.05,
spacing_y * 0.05)
cx = margin_x + col_idx * spacing_x + rng.uniform(-spacing_x * 0.05,
spacing_x * 0.05)
r = base_r_global * (1.0 + rng.uniform(-colony_size_variation,
colony_size_variation))
# Core
core_mask = _radial_colony_mask(plate_h, plate_w, cy, cx, r * 0.6, rng)
# Filaments
local_density = filament_density * rng.uniform(0.9, 1.1)
fil_mask = _filament_mask(
plate_h, plate_w, cy, cx, r * 0.6,
density=local_density,
reach_factor=filament_reach,
rng=rng
)
# Combine
combined_mask = np.maximum(core_mask, fil_mask)
img = _screen_blend(img, combined_mask[..., None], col_rgb)
return _quantize(img, bit_depth)
# --- Loaders ---
[docs]
def load_synth_yeast_plate():
"""Returns a phenotypic.GridImage of a synthetic plate with the colonies detected"""
from skimage.io import imread
dirpath = __current_file_dir / "synthetic_test_plate"
image = phenotypic.GridImage.imread(
filepath=dirpath / "yeast_plate_rgb.png"
)
image.objmap[:] = imread(dirpath / "yeast_plate_objmap.png")
image.name = "Synthetic96PlateWithObjects"
return image
def load_synth_filamentous_plate():
from skimage.io import imread
dirpath = __current_file_dir / "synthetic_test_plate"
image = phenotypic.GridImage.imread(
filepath=dirpath / "filamentous_plate_rgb.png"
)
image.objmap[:] = imread(dirpath / "filamentous_plate_objmap.png")
image.name = "Synthetic96PlateWithObjects"
return image
