Source code for phenotypic.detect._yen_detector
from __future__ import annotations
from typing import TYPE_CHECKING
if TYPE_CHECKING:
from phenotypic._core._image import Image
from skimage.filters import threshold_yen
from skimage.segmentation import clear_border
from ..abc_ import ThresholdDetector
[docs]
class YenDetector(ThresholdDetector):
"""Detect colonies by maximising the correlation between the original and binarised image.
Compute a threshold that maximises the squared correlation coefficient
between the original intensity image and its binarised version. Handles
skewed histograms reliably, offering a middle ground between variance-based
(Otsu) and entropy-based (Li) criteria. For a full comparison of detection
strategies see :doc:`/explanation/detection_strategies_compared`.
Best For:
- High-contrast plates with clear intensity separation between colonies
and agar background.
- Images with skewed histograms where one pixel class is substantially
larger than the other.
- Exploratory analysis when unsure whether a variance-based or
entropy-based criterion better fits the plate histogram.
Consider Also:
- :class:`OtsuDetector` for a faster variance-based threshold when the
histogram is balanced and clearly bimodal.
- :class:`LiDetector` when the histogram is low-contrast or unimodal
and entropy-based separation is more appropriate.
- :class:`TriangleDetector` when colonies are very sparse and the
histogram is strongly background-dominated.
Args:
ignore_zeros: Exclude zero-intensity pixels from the histogram before
computing the threshold. Enable for plates with black borders or
masked regions. Default: False.
ignore_borders: Remove colonies touching image edges via
``clear_border()``. Recommended for grid-based colony counting
to eliminate partial colonies at plate boundaries. Default: True.
Returns:
Image: Input image with ``objmask`` set to the binary colony mask and
``objmap`` set to labeled connected components.
Raises:
ValueError: If threshold computation fails due to a degenerate
histogram with insufficient intensity variation.
References:
[1] J. C. Yen, F. J. Chang, and S. Chang, "A new criterion for
automatic multilevel thresholding," *IEEE Trans. Image Process.*,
vol. 4, no. 3, pp. 370--378, 1995.
See Also:
:doc:`/tutorials/notebooks/02_detecting_colonies` for a step-by-step
tutorial on basic colony detection.
:doc:`/how_to/notebooks/choose_detection_algorithm` for guidance on
selecting the right detector for your plate images.
:doc:`/explanation/detection_strategies_compared` for an in-depth
comparison of all thresholding strategies.
"""
ignore_zeros: bool = False
ignore_borders: bool = True
def _operate(self, image: Image) -> Image:
"""Binarizes the given image gray using the Yen threshold method.
This function modifies the arr image by applying a binary mask to
its detection matrix (`detect_mat`). The binarization threshold is
automatically determined using Yen's method. The resulting binary
mask is stored in the image's `objmask` attribute.
Args:
image (Image): The arr image object. It must have an `detect_mat`
attribute, which is used as the basis for creating the binary mask.
Returns:
Image: The arr image object with its `objmask` attribute updated
to the computed binary mask other_image.
"""
enh_matrix = image.detect_mat[:]
nbins = 2**image.bit_depth
mask = image.detect_mat[:] >= threshold_yen(
enh_matrix[enh_matrix != 0] if self.ignore_zeros else enh_matrix,
nbins=nbins,
)
mask = clear_border(mask) if self.ignore_borders else mask
image.objmask = mask
return image
# Set the docstring so that it appears in the sphinx documentation
YenDetector.apply.__doc__ = YenDetector._operate.__doc__